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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been noticed in several tumors, which leads to chromosome instability, and thus could lead to tumorigenesis. This protein was located to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a important job in promoting mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are actually explained. Two added splice variants had been reported but specific nucleotide sequence facts is not really yet offered.
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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide ordinarily contains the epitope acknowledged because of the antibody.
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This tutorial outlines further attainable optimization of mobile and immunoassay parameters to guarantee the very best success are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to ensure the absolute best benefits are...
Antibodies certain to the blocking peptide not bind on the epitope around the goal protein. By comparing the staining within the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Goal Details Cyclin E belongs towards the extremely conserved cyclin family members, whose customers are characterized by a dramatic periodicity in protein abundance in the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead for the temporal coordination of each mitotic celebration. Cyclin E forms a posh with and features like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S section boundary and it is degraded as cells development by way of S section.
The measurement of protein phosphorylation is a useful tool for more info measuring the modulation of receptor activation by both equally antibodies...
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